COST Training school Aveiro, 2. - 6. 4. 2018

  • IMG 7372
  • IMG 7384
  • IMG 7391
  • IMG 7405 Church of Saint Ildefonso
  • IMG 7415
  • IMG 7421
  • IMG 7425
  • IMG 7430
  • IMG 7450 Preparing conidial suspension in PTB
  • IMG 7452
  • IMG 7453 Filtering of the suspension to separate the clean microconidia from hyphae and other debris.
  • IMG 7459
  • IMG 7466 Estimating the concentration of the microconidia in the medium by Bürker chamber
  • IMG 7474 Jorge is explaining where to inoculate the pine sapling.
  • IMG 7487 Eva, making the inoculation wound.
  • IMG 7479 Inoculation wound in the bark. Can you find it? It is in the level of the tip of her nose.
  • IMG 7484 Inoculated by 10 ul of conidial suspension of 1000 spores/ml.
  • IMG 7492
  • IMG 7485 Inoculum covered by parafilm
  • IMG 7488
  • IMG 7483
  • IMG 7489
  • IMG 7493
  • IMG 7497 Control and inoculated plant.
  • IMG 7500 Visible necrosis above the point of inoculation.
  • IMG 7517
  • IMG 7510
  • IMG 7512
  • IMG 7521 Aveiro
  • IMG 7523
  • IMG 7526 Aveiro - the Venezia of Portugal
  • IMG 7527
  • IMG 7528 Preparing the needles for cell wall permeability test
  • IMG 7529 Washing by water in microtube.
  • IMG 7530 Transfering to falcon with water.
  • IMG 7533 Measuring the electrolyte conductance
  • IMG 7534
  • IMG 7544 Longitudinal cut of the infected sapling.
  • IMG 7538
  • IMG 7549 Measurement of the PPC necrosis
  • IMG 7552 Most of the organizing team and Bora
  • IMG 7553 PPC necrosis under binocular magnifier
  • IMG 7555 Inoculation point of the control sapling
  • IMG 7557 Measuring the fluorescence with a machine made in BRNO
  • IMG 7556
  • IMG 7558 Needles of inoculated saplings have clearly lower fluorescens
  • IMG 7559 Small fluorescence detector mainly for broadleaves.
  • IMG 7560
  • IMG 7565 Cofeebreak
  • IMG 7570 Carbon dioxide detector - detecting the photosynthetic activity.
  • IMG 7594 Photosynthetic activity is provoked by the ph. most active spectrum of radiation.
  • IMG 7597 Smaller CO2 detector for broadleved leaves.
  • IMG 7575 Pressure chamber for water potential assay
  • IMG 7576
  • IMG 7580 Sealing the sapling in the center of the cap of the chamber.
  • IMG 7581
  • IMG 7584 Regulated preasure on the upper part of the seedling is pushing out the droplets of water on the cut, which is followed by a magnifier.
  • IMG 7587
  • IMG 7604 Taking needles for the analyzes
  • IMG 7602 Liquid nitrogen for disruption of nedles for the proline and enzymes assay.
  • IMG 7608 Disruption of needles by mortar and pestle.
  • IMG 7623
  • IMG 7624
  • IMG 7622 Adding of buffer
  • IMG 7609 "Pesto" made of pine needles of the non-inoculated tree.
  • IMG 7627
  • IMG 7615 Perfectly homogenized needles with disrupted cell walls
  • IMG 7620 "Pesto" after centrifugation
  • IMG 7633
  • IMG 7610
  • IMG 7612
  • IMG 7616 Glass grip :-)
  • IMG 7619 Look at the fool, he does it as well :-D
  • IMG 7625
  • IMG 7640 Rich conidial suspension of our working group - great result!
  • IMG 7647 Proline assay - taking the discoloured buffer for spectrometry measurement
  • IMG 7653 REsult is visible by naked eye - difference between control and inoculated plants is striking
  • IMG 7656 Visit on the other side - Costa nova
  • IMG 7657
  • IMG 7658
  • IMG 7659
  • IMG 7661
  • IMG 7664
  • IMG 7666
  • IMG 7678
  • IMG 7685
  • IMG 7686
  • IMG 7689
  • IMG 7692
  • IMG 7693
  • IMG 7710 Social dinner
  • IMG 7722 Party after the dinner
  • IMG 7729
  • IMG 7750
  • IMG 7761 Some completely different people :-D
  • IMG 7764 On my way back - thanks to missed flight I had opportunity to visit Porto more. I was amazed by this train station!
  • IMG 7765
  • IMG 7767
  • IMG 7769
  • IMG 7771
  • IMG 7772
  • IMG 7780
  • IMG 7781
  • IMG 7783
  • IMG 7786
  • IMG 7793
  • IMG 7801
  • IMG 7805